Converts region mapping to genomic mapping
This takes a BAM file that has been mapped to a genomic region and converts
the mapping to genomic coordinates. This can be used to convert reads mapped
against a junction library or targeted resequencing back to genomic
coordinates. The names of the reference sequences should be named:
chrom:start-end (0-based start). If there are gaps (junctions), they should
be named: chrom:start-end,start-end,etc...
In this is the case, this script will ensure the proper conversion of
reference, start position, and CIGAR alignment.
Example 1:
chr1:1000-2000 20 50M
converted to:
chr1 1020 50M
Example 2:
chr1:1000-1050,2000-2050,3000-4000 25 100M
converted to:
chr1 1025 25M950N50M950M25M
Usage: bamutils convertregion {-overlap} in.bam out.bam [chrom.sizes]
(Note: A samtools faidx file can be used for the chrom.sizes file.)
Options:
-f Force overwriting an existing out.bam file
-unmapped Keep all unmapped reads in the output file (including invalid junction reads)
-overlap N Require that all reads must overlap a splice junction
by N bases. This also removes unmapped reads. [default 4]
Set to zero to allow all reads.
-validateonly Don't convert the reference and position, just confirm that
the reads correctly overlap a junction. Any reads that don't
overlap a junction will not be written to the out.bam file.
If -validateonly is set, then the chrom.sizes file isn't
required.