Finds regions of unusual deletions (CLIP-seq)
Given a set of BAM files, we search for areas where there are an unusual
amount of deletions. For CLIP-Seq, this can be an indicator of the location
of protein-RNA interaction.
Output is either a BED file or a FASTA format file containing these hotspots.
Only unique regions are returned across all files.
See: Zhang and Darnell, Nature Biotechnology (2011)
doi:10.1038/nbt.1873
pmid:21633356
Usage: bamutils cims {opts} in.bam {in.bam...}
Options:
-fasta ref.fa Ouput in FASTA format (requires reference genome.fa)
[default: BED output]
-flanking N The number of flanking bases on either side to report
(FASTA output only) [default: 12]
-cutoff N Cut-off % for deletions - if the % of reads that
include a deletion at a position is higher than this
number, the fragment is reported (0->1.0)
[default: 0.1]
-ns Don't take the strand of the read into account
-window N The maximum length of a deletion window
[default: 20]